BIOL 112 Lecture Notes - Lecture 5: Semiconservative Replication, Pyrophosphate, Primase

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BIOL 112 Full Course Notes
27
BIOL 112 Full Course Notes
Verified Note
27 documents

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Dent lecture 5: discovery and replication of dna. Extract lipids and proteins with organic solvent (e. g. phenol) R bacteria acquired the heritable characteristics of s bacteria, that is the genetic material transferred from s to r. The live r take up the genetic material and become the live s! Oswald avery uses griffith"s assay to identify the transforming principle. > dna is the substance that is able to convert r to s. Dna is the transforming principle or the genetic material. Label protein with radioactive sulphur and dna with radioactive phosphate. They took a virus that consist of a dna core surrounded by a protein coat and radioactively labelled the dna with. Allowed the virus to infect bacteria and then mix in a blender, then centrifuge. Effect: the protein coat was sheared off the bacteria and the dna stayed with the bacteria. Infected bacteria now produced more virus which contained 32p (dna) but almost no 35s(protein).