BIOL 200 Lecture Notes - Lecture 31: Functional Genomics, Microrna, Small Interfering Rna
Document Summary
Argonaute protein binds fragments of dsrna, forming risc (rna-induced silencing complex) Thru atp hydrolysis, risc can perform its helicase activity & unwind the sirna. Risc uses ssrna (single-stranded rna) as guide to target the complex to complementary cellular. Rnas, associating through watson-crick base pairing, resulting in cleaved transcripts. If lin-14 sequence is gone, lin4 doesn"t have its mrna target, animal will never escape larva stage (peter pan gene) Requires dicer to chop the dsrna to generate sirna, which is then bound by argonaute proteins and unwound by risc (very similar to rnai pathway) The same dicer works on dsrna and mirna, that"d eventually give rise to rnai mirna works in cell to regulate mrnas (inhibit translation; regulates 50% of genome) We don"t know the functions of >50% of the genes. Genes that cause embryonic lethality & sterility are involved in basal cellular function: translation, transcription, splicing transcription, splicing .
