BIOL 200 Lecture Notes - Lecture 26: 28S Ribosomal Rna, Ribosomal Rna, Release Factor
Document Summary
Ef1 alpha, bound to gtp, will interact w/ the a site. If the codon and anticodon don"t match, the ef1 -gtp complex will go into a site, wiggle around a bit, then leave. Only when you get a properly matched codon, the gtp will hydrolyse, allowing the correct trna to bind. Ef1 -gdp leaves the trna, allowing the new aa to come close to the aa in p site. Through activity of a rna, not a protein! A ribozyme -- rna molecule that is enzymatic. Peptidyltransferase activity: depends on 23s rrna (part of the. Prokaryotic ribosome; same applies to 28s rrna in eukaryotes) 23s rrna catalyzes peptide bond formation (no protein required) Once peptide bond is formed, ef-2 bound gtp is recruited, and hydrolyzed. Its hydrolysis leads to conformational change in ribosome, causing translocation of trna molecules into e and p sites respectively. Now, growing peptide chain in at the p site.
