BIOL 200 Lecture Notes - Histidine, Conformational Change, Amphoterism
6 Apr 2012
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Document Summary
As we have seen over throughout this course, proteins have a wide variety of functions which depend on their structure. Some specific domains of proteins can carry out independent functions: an example is the dna binding domain found in transcriptional activators such as homeodomain proteins. This causes the mrna to have the gfp mrna causing the translation of a fusion protein by the ribosome. During transcription, rna polymerase ii (or whichever is used) skips the stop site of the desired proteins gene and performs read- through right to the end of the gfp gene. X-ray diffraction and crystallography are two techniques widely used to accurately determine the structure of proteins. High concentrations of a purifies proteins, perhaps made from bacterial overexpression, tend to form crystal lattice structures: this structure corresponds to the protein"s lowest energy state. Complex programs are able to take this scatter data and determine the function of the protein through prediction of electron density.
