BIOL 201 Lecture Notes - Absorption Spectroscopy, Carbon Fixation, Light-Independent Reactions

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Photosynthetic organisms capture light energy (the ultimate source of biological energy) and use it to: acceptor molecules. Transfer h atoms from acceptors to carbons derived from co2 to form glucose (process known as carbon fixation: net reaction: Reduction of co2: oxidation of h2o absorption of light. 2 h2o + 2 nadp+ + 3 adp + 3 pi. O2 + 2 h+ + 2 nadph + 3 atp. Co2 + 2 h+ + 2 nadph + 3 atp. Ch2o + h2o + 2 nadp+ + 3adp + 3 pi. Later and in a subset of eukaryotic: double membrane organelles with own genome diagnostic transferred to the nucleus. Light absorption and energy transformation can be measured via: Chlorophyll a: peaks at 430 and 650 nm. E. g. levels of o2 released by by wavelength. Returns to ground state by emitting a less energetic photo (fluorescence) or heat.

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