Neurospora is easy to grow, simple carbon and very basic elements are enough for the wild types to
Mutants shouldn’t be just sick, they need to have a simple pathway disrupted to be controlled. Bred
with a normal wild type to create a functional fruiting body to ensure that it’s a desired mutant. TO test
what’s going on, we put it on regular medium, and then slowly take away each nutrient until we find out
what would prevent further growth.
For a metabolic pathway, the mutations would shut down a certain enzyme, and testing would be able
to show exactly which of the enzymes were inactivated. The further downstream the mutation, the less
possible the materials that could sustain the growth. Beadle and Tatum received a Nobel Price in 1958
for their 1 gene 1 enzyme discovery.
Gene Complementation Testing
Testing of multiple mutants creating hybrid mutants of the same allele (different type of mutation on
the same allele, now 2 copies of the allele for the same gene differs in their mutations). This could be
used to show haploefficiency. If the 2 mutations are on different genes on the same pathway, and a
normal wild type pheno