BIOL 300 Lecture Notes - Small Interfering Rna, Intron, Drosha
15 Dec 2012
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Document Summary
The work that led to the nobel prize related to small rnas was based on knocking out genes through injection of double stranded rna molecules (which is commonly done now) It took years of research to realize that this mechanism is actually the same mechanism as the lin4 mechanism. This all began with the idea that you could knock out gene expression by introducing antisense rna: the idea was that the antisense. Rna would bind and inactivate the mrna by preventing translation. Additionally, you could conduct an rna in-situ hybridization technique to display the amount of gene expression in the cells (you could also do a northern blot fo achieve the same results, except the rnas are result on a gel) They then carried out experiments to see how much of the correct dsrna you needed to observe the full phenotype: they did this by incrementally injecting less and less dsrna until they no longer observed a phenotype.
