BIOL 300 Lecture Notes - Lecture 10: Ubiquitin, Multiprotein Complex, Nucleosome

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Transcription elongation by pol ii is helped along by a series of elongation factors: Different classes of elongation factors have been identified in recent years, some are direct while others are indirect. Factors which regulate the rate of elongation through chromatin (no effect on transcription on. Tfiis, often called iis, is a ubiquitous protein found in all eukaryotes from yeast to man; there are also some bacterial counterparts called grea and b. Pol ii has a tendency to pause or completely arrest at certain dna sequences, followed by a backtrack which leaves the. 3"oh of the nascent mrna out of position relative to the active site: rna pol ii will now be trying to add a nucleotide where rna is already present. Tfiis helps pol ii overcome this by stimulating a latent pol ii endonuclease activity and creating a new 3"oh in the mrna which is positioned correctly in the active site.

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