BIOL 300 Lecture Notes - Lecture 26: Alanine, Conformational Change, Chromosome
5 Mar 2018
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Growth factors that can be used to fish out the receptor because of its very affinity to its receptor: 2. General application: fuse a tag to the protein of interest you want to purify and then use antibody that bind to that tag with high affinity. How to analyze the function of kinase substrates: when an amino acid gets phosphorylated, the protein undergoes a conformational change, 1. Receptor sequestration: moving the entire ligand-receptor complex into endosomes where it will be recycled or degraded, 2. Receptor inactivation: some other proteins can bind to the receptor and deactivate it. Mutagenizing (introduce at least one lethal mutation: you now have a large collection of mutants, 2. Rough mapping of the chromosome: for example you now know there are 400 mutations on chromosome 2, 3. Complementation: when you randomly mutagenize, you often generation multiple mutations in the same gene.
