BIOC 300D2 Lecture 1: Lecture 1

• School: McGill University
• Department: Biochemistry
• Course: BIOC 300D2
• Professor: Jose G.Teodoro

Document Summary

Optics and enzyme kinetics: related topics since when you follow reactions, you use spectrophotomer (optics as a way of readout of enzyme reaction) Narrow window that is the optical spectrum of light that we can see. In biochemistry, we extent the spectrum to ultraviolet radiation (passed blue). Calculated usually as transmittance, which is defined as the fraction of light passed = how much light goes in (incident) and how many goes out (exiting) Transmittance always between 0 (opaque) and 1 (clear) Absorbance is -log(t) analog to ph equation ph = -log[h] Log scale useful properties when you need to multiply them. In lab, take a cuvette with path length filled with fluid and light goes through. Path length: denoted d, width of cuvette (usually 1cm) Naming parts of uv-vis opticak spectrometer: used to measure visible and (near) uv light absorbance. Monochromator or slit (filter): selecting out one wavelength of light you are going to go through.