BIOT 505 Lecture Notes - Lecture 2: Hematopoietic Stem Cell, Zinc Finger, Bone Marrow
31 Mar 2018
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Document Summary
Selected topics in biotechnology: non-transgenic, i. e. bone marrow, versus transgenic, mouse is the most important animal in stem cell research. Fuse mouse and human embryo: 2013, introduced crispr/ cas technology. Multipotent: cells become more differentiated, specific cell type, i. e. hematopoietic stem cell for blood cells, but you can"t go back and turn into neurons, skeletal system, etc. Unipotent: muscle cells, one divides and the other becomes a stem cell. So we know what a differentiated cell is. Ips, and put them back into the oocyte to make that first embryo. Not necessarily efficient with only 1 of 250 working: technology still exists for important animals, mouse: inbred genotype is almost identical, outbreed is like with everyone, mosaic only some will get the integrated dna. Idea now is to implant things to correct the genome in a very precise way: normally if no intron in cdna, it will be suppressed.
