FDSC 300 Lecture Notes - Lecture 5: Solvent, Petroleum Ether, Mouthfeel
20 Oct 2017
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Mostly tg glycerol backbone w/ 3 fa, connect w/ ester linkages (fa: 4-24c) Simple lipids (wax or fa), compound lipids (phospholipids, etc), derived lipids (derivatives, sterols, long chain alcohols, etc) Can separate based on solubility protein/carb soluble in water, lipid is not. Diff types of lipid will have diff range in solubility. Crude fat = nonpolar lipids, mostly tg (polar lipids will not be counted) Total lipid = nonpolar + polar -> wet extraction (babcock, majonnier, bligh&dyer, berber) Crude fat = polar only -> dry extraction (sox, goldfish) Extract fat using nonpolar solvent -> evaporate solvent -> crude fat remains: solvents: hydrocarbons, petroleum ether (very non polar); diethyl. Bad); n-butanol (but oh group makes it more polar) Dry extraction method: (may need to break down sample w/ hcl first) Discontinuous: need lot of solvent: use fixed amount, need 1:10 ratio sample to solvent (may not extract completely, soxhlet method: boiling solvent -> condense down onto sample -> siphoned.
