LSCI 204 Lecture Notes - Lecture 21: Retrotransposon, Transposase, Dna Ligase

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The trumpet lily (lillium longiflorum) has about 90 billion base pairs of dna in its genome, almost 30 times the amount found in humans. Transposable elements: sequences that can move about the genome. Features: flanking direct repeats, terminal inverted repeats. Flanking direct repeats are generated when a transposable element inserts into dna: short flanking direct repeats from 3 to 12 bp long are present on both sides of most transposable elements. Are not a part of a transposable element and do not travel with it. Rather, they are generated in the process of transposition, at the point of insertion. Many transposable elements have common characteristics: transposable elements are terminal inverted repeats. Sequences from 9 to 40 bp in length that are inverted complements of one another (a) For example, the following sequences are inverted repeats: Flanking direct repeat: the two sequences are not simple inversions.

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