LSCI 204 Lecture Notes - Lecture 22: Orites, Lac Operon, Cloning
8 Feb 2018
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Recombinant dna technology (genetic engineering: techniques for locating, isolating, altering and studying dna segments. The molecular genetics revolution: biotechnology: the use of these techniques to develop new products. Amplifying dna fragments with the polymerase chain reaction (pcr) The pcr reaction: taq polymerase: stable dna polymerase at high temperature, reverse-transcription pcr. Limitations of pcr: prior knowledge of target dna, contamination, accuracy, amplified fragments are less than 2kb. Applications of pcr: real-time pcr: quantitatively determining the amount of dna amplified as the reaction proceeds, the polymerase chain reaction is used to amplify even very small samples of. 90 100 c to separate the two strands. quickly cooled to 30 65 c to allow short single- strand primers to anneal to their complementary sequences. heated to 60 70 c; Each time the cycle is repeated, the amount of target dna doubles. To carry out pcr, we begin with a solution that includes the target dna,
