MIMM 211 Lecture 38: Virus pathogens + review
You are studying the calnexin protein, To study the calnexin protein more, you decide to mutate the amino acids KKRR found on the C-terminal end in calnexin to amino acids AAAA, to see how this might affect itâs function.
A. What is the original function of calnexin, and where does it reside? (2 pts)
B. First you test the function of calnexin in a test tube, and you find that it doesnât lose itâs original activity. However, cells that contain only the mutated form (AAAA) of calnexin do not function correctly. Please explain (be specific) how the mutation (AAAA) is affecting Calnexinâs role in the cell. (3 pts)
C. Please explain how the mutation (AAAA) might affect other proteins that enter into the endomembrane system. (3 pts)
Deduce the phenotypic proportions in the progeny of thefollowing crosses of autotetraploids. Assume that the fourhomologous chromosomes of any one type pair randomly two-by-two andthat only one copy of the a+ allele is necessary for the wild-typephenotype. A. a+a+aa x aaaa B. a+aaa x aaaa C. a+aaa x a+aaa D.a+a+aa x a+aaa