MIMM 211 Lecture Notes - Lecture 10: Superoxide, Turbidity, Uracil
13 Dec 2018
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Document Summary
When you put bacteria in non-optimal ph, they will either not grow very well or not at all. Not the gap at the bottom of the table. Oxygen produces toxic molecules reactive oxygen species (ros) If we do not detoxify these molecules, the dna, proteins and lipids will be attacked by them leading to degradation. Superoxide molecules can then be transformed into hydrogen peroxide (more dangerous) Protective enzymes of obligate aerobes and facultative anaerobes. Sod transforms superoxide into o2 and h2o2 where the h2o2 can be taken care of by other enzymes. Detoxification of h2o2 is done by two enzymes: catalase forms h2o and o2, peroxidase forms h2o and nad+ When these enzymes are not present, the bacteria cannot cope with oxygen in their environment. We mix liquid agar with our bacterial cells and then let it solidify. In the tube, the highest o2 concentration will be at the top (it will decrease as you descend the tube)
