MIMM 214 Lecture Notes - Lecture 28: Rna Splicing, Plasma Cell, Immunoglobulin D
17 Apr 2017
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Generation of tcr diversity & a return to antibodies. Combinatorial diversity of tcr: vj in chain, vdj in chain, combination of and chain - another layer of diversity. Similar mechanism to bcr: v, d, and j segments are flanked by rss, rag-1/2 recognize these sequences - same ones in bcr recombination process. Tdt adds non coded nucleotides in the joining regions. Tcrs have cdrs as well: 3 cdrs per chain, cdr3 is a particularly important source of diversity. These are the sites with most diversity: what is the functional significance of the highly variable nature of cdr3 compared to the other. Cdrs: cdr3s are centrally located and are the ones mainly responsible for peptide binding; the other cdrs are mainly responsible for recognizing the mhc structure, there is some overlap. Secreted and transmembrane mrna are the result of rna splicing as well: the primary rna transcript has both for tm and secreted, until it goes through splicing.
