PHAR 303 Lecture 8: PHAR 303 lecture 8
15 Feb 2018
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Document Summary
Omics used to just be toxicogenomics, but there are many more methods that have been developed over the years. New measures: gene expression: dna microarrays, rt pcr, sequencing, protein expression: western blot, electrophoresis, chip. Proteomics is becoming a more important part of the field. Determine whether gene, rna, protein, or metabolite expression profiles ( signatures ) can serve as markers to predict toxicity: try to find if a particular toxicant has a certain signature. Epigenomics: the study of all epigenetic modifications all non-sequence effects: e. g. reversible modifications on a cell"s dna or histones that affects gene expression without altering the dna sequence. If you are using a pure cell line, this doesn"t tell you what is happening in the whole animal. Transcriptomics requires purified cell populations: the same is true for proteomics. Proteomics: the study of all the proteins of a cell or tissue: the advances in the past 5 years with gc-mass spec. have been dramatic.
