BIOCHEM 2B03 Lecture Notes - Helicase, Dna Ligase, Dna Supercoil

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There are 2 replication box come out from oric. Dna synthesis using stdna as a template. Both strands move in - same direction but in a different fashion. 3" oh group is attached to the alpha phosphate on the deoxy-ntp. Gamma- beta-- alpha (attached to 3"oh on the upper sugar) phosphate. Pyrophosphatase : used to label any enzymes that can remove any kind of phosphate. Special to hydrolyze the pyrophosphate at the phosphodiester linkage. E. coli dna polymerase: 3 common : pol 1, pol ii and pol iii (remember only pol 1 and pol 3, pol 1: dna repair, dna synthesis start from making rna as a primer. These donut go through the single stranded dna. Try to get polymerase to stay on and continue it"s job: tao unit is the linker to bring the two polymerase together, memorize the whole table 28. 2, pol iii holoenzyme.

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