Class Notes (836,587)
Canada (509,861)
Biochemistry (232)
BIOCHEM 2EE3 (157)
Lecture 13

Biochemistry 2EE3 Lecture 13.docx

7 Pages
Unlock Document

Karen Mc Garry

Biochemistry 2EE3 Lecture 13 • • • Channel allows passive transport • Pumps allow active transport, use a lot ofATP to transport against concentration gradient • • Two types of passive transport • Carrier: hydrophobic exterior, hydrophilic binding site which diffuse through membrane – called ionosmall molecules which pass through membrane, goes according to concentration and electrochemical • Channel forming • • Synthesized by bacteria • • Malfunctioning of heart associated with malfunctioning ion channel proteins which inhibits electrical stimulus of heart • Without knowing 3D structure of ion channels, do not know how they work • Crystallized ion channel from bacterial worm because it was much simpler than human ones • Source of potassium channel is from KcsAbacteria who live in high temperature • Synthesized protein from genes and predicted which parts should be interior and transmembrane • Cut off everything except transmembrane part • Four subunits, in closed conformation • Ions do not go through ion channels without water, a lot of energy must be invested to remove water molecule • Hydrations costs a lot of energy about 100kcal, energy larger than the energy of some chemical bonds • Water is not replaced bycause there is no oxygen • Red areas are negative charges, hydrated ions can byt in middle, can be easily replaced by oxygen atoms • • • Only one channel discussing in detail – favourite proteins, on midterm • Details of selectivity filter, which discriminates potassium ions from all others by • Green: K, red: O • Binding site #1: 8 O atoms around K, costs nothing for K to get rid of H2O because surrounded by O, transferred
More Less

Related notes for BIOCHEM 2EE3

Log In


Join OneClass

Access over 10 million pages of study
documents for 1.3 million courses.

Sign up

Join to view


By registering, I agree to the Terms and Privacy Policies
Already have an account?
Just a few more details

So we can recommend you notes for your school.

Reset Password

Please enter below the email address you registered with and we will send you a link to reset your password.

Add your courses

Get notes from the top students in your class.