BIOCHEM 2BB3 Lecture Notes - Lecture 7: Nucleophile, Enzyme Kinetics, Choline

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The reaction rate = velocity (v), which describes the rate of disappearance of the substrate (s) or rate of appearance of the product (p) With a constant [enzyme], changes in [su(cid:271)st(cid:396)ate] (cid:272)ha(cid:374)ges the (cid:396)ea(cid:272)tio(cid:374)"s (cid:448)elo(cid:272)it(cid:455) i(cid:374) a hyperbolic fashion: hyperbolic; enzyme physically binds to the substrate to for es (enzyme-substrate) complex. Low [substrate], the rate increases quickly until the enzyme becomes saturated. The order of the reaction is dependent on the number of substrates. Second order: 2 substrates = bimolecular; m-1s-1 k1 is a second order reaction and k2 is a first order reaction. Michaelis-menten equation, assume [s] >> {e] k2: know velocity and [es] Velocity: synthetic substrate that is converted to a light-absorbing/fluorescent product: velocity is the rate of appearance of the product monitored by a spectrophotometer or fluorimeter. [es]: difficult because it depends on the rate of its formation (k1) and its decomposition (k-1) Rate of [es] = k1[e][s] k-1[es] k2[es]

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