BIOCHEM 2EE3 Lecture Notes - Lecture 6: World Federation Of Trade Unions, Intron, Thermodynamic Cycle

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Transcription has 3 phases: initiation, elongation, termination. Dna sequence encodes an number of key elements: Initial elements to direct rna polymerase biding to the dna at the start of the sequence. Termination elements that direct rna polymerase to stop transcribing the dna. A sequence of dna to which rna polymerase binds and initiates transcription. 10 and -35 regions are involved in tight binding to rna polymerase. 5" to 3" stra(cid:374)d is k(cid:374)o(cid:449)(cid:374) as the se(cid:374)se stra(cid:374)d a(cid:374)d is (cid:374)ot used (cid:271)y rnapol as the template. (cid:373)rna is (cid:373)ade i(cid:374) the 5" to 3" dire(cid:272)tio(cid:374) the catalytic core of the active rnapol cuts into the protein itself. Mrna chain growth begins with the binding of dna template strand specified rntp. Active site of rnap contains the unwound dna double helix. During elongation the core rnap enzyme moves along the duplex dna template unwinding the dna template exposing the single strand antisense template for base.

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