BIOCHEM 2EE3 Lecture 4: Module 2 video 2

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1. 8 pka and pkb the weak world. 1. 10 consequence of ph changes on biomolecules: when you go from an acidic (or low) ph to a basic (or high) ph, the surface charge character of this biomolecule changes from mostly blue (positive) to mostly red (negative) This information can be plotted on a graph. 1. 13 titration curve of 1. 0m formic acid with naoh: x-axis: the amount of oh ions added (in this case we would graph the volume of naoh, y-axis: the ph of our solution. Example: we want to titrate a 1m solution of formic acid with sodium hydroxide. We also know the pka of formic acid by looking it up on table 2. 6. Solve for x and you get 1. 33 x 10-2 m: subtract this number from our initial concentration of 1m we obtain 0. 9867m, this is approximately 1m rounded up.

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