BIOCHEM 2EE3 Lecture Notes - Lecture 3: Isoelectric Point, Biomolecule, Ideo

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Only substance on earth that can be found naturally in all 3 states. Expands as a solid neutral ph universal solvent of life therefore most reacions occur in aqueous soluions. 70% of the weight of a cell = water forms a laice around sodium + chloride. Arrhenius theory acids = hydrogen ions (h+) while bases produce hydroxide ions (oh-: acid plus base = neutral because they produce water, hcl + naoh nacl + h20. Bronsted-lowry theory acids proton donors , while bases = proton acceptors: strong acids dissociate into a proton and a weak conjugate base (h = acid ; when dissociated) Very minimal change in ph during the bufer region. Some have muliple ionizable groups; creaing muliple bufer regions. If you want study a reacion at ph 4; the best bufer would be one with a pka around 4: the bufers work opimally around their pka.

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