BIOCHEM 2EE3 Lecture Notes - Lecture 10: Dihydrolipoamide Dehydrogenase, Pyruvate Carboxylase, Pyruvate Dehydrogenase

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2ee3- module 10: citric acid cycle and lipid metabolism. Glucose undergoes glycolysis (cid:0) pyruvate undergoes oxidation in the mitochondria (cid:0) acetylcoa undergoes citric acid cycle (cid:0) co2. Pdh, pyruvate dehydrogenase: catalyzes the oxidation of pyruvate (cid:0) acetycoa. Citric acid cyle has 8 reactions: acetylcoa (cid:0) co2 + 3 nadh + fadh2 + Gtp: in etc, the potential energy in nadh and fadh2 will beused to make atp. Pdh reaction and tca cycle occurs inside the mitochondria. Mitochondria is where most of the atp is generated from in the cell = powerhouse of the cell. It has two membranes: outer: surrounds the surface, inner: invaginated- to form cistae- to increase surface area, matrix: space inside the cell, formed by the inner membrane, intermembrane space: space between the inner and outer membranes. Free thiol group on one end: the reactive end; Reduced form of coenzyme a is known as coash. Pyruvate + o2 (cid:0) co2 + h2o: decarboxylation of pyruvate acetylcoa a.

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