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BIOCHEM 3H03 Lecture 26: BIOCHEM 3H03 LECTURE 26

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13 Sep 2018

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McMaster University

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Analytical techniques: analytical test- osmolality o osmolality(plasmaandurine) o. Osmometry= technique for measuring the concentration of solutes that contribute to osmotic pressure of a solution o. Colligativeproperties=propertiesthatdependonnumberandnotthe nature of the dissolved particles ex. Osmotic pressure, elevation of boiling point, freezing point depression, vapor pressure lowering are all colligative properties: how to measure freezing point depression. Quickly cool the sample to below the freezing point (supercool, still in liquid form) Solution reaches equilibrium where freezing and thawing are equal accurately measure this temperature. Specific gravity uring refractometry, light will refract different with different densities* Couldthisbemethanolorethyleneglycolpoisoning?isthereanother osmotically active particle there that we are not calculating o. Calculatedosmolality,withequation=285mosmol o measured osmolality= 312 mosmol o there is a osmolar gap of 27, which suggests presence of abnormal osmole present o patient airlifted to hospital where [ethylene glycol] was found to be. Measurement of na and k flame emission spectroscopy: metalionswillemitlightofspecificwavelengthwhen given enough energy.

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Related Questions

DETERMINATION oF MOLAR EXPERIMENT 23 MASS FROM FREEZING POINT DEPRESSION EQUIPMENT AND CHEMICALS: Equipment 600 mL beaker Chemicals Cyclohexane (C Stir plate 100 ml. graduated cylinder Clamp Nonvolatile Unknowns Freezing point apparatus Spin vane 0.1 C Thermometer INTRODUCTION: When a solute is introduced into a pure solvent the newly formed solution will have certain physical properties that are from the solvent. As a of the presence of the the pressure of the solution will be lower than that of the pure solvent. As the vapor pressure is lowered there is a resulting increase in the amount of energy required to reach the boiling point of the solution. Conversely, the solution must cool to a lower temperature before a state change from liquid to solid occurs. These changes in physical properties vapor pressure lowering, boiling point elevation and freezing point depression are collectively referred to as colligative properties. These colligative properties, which also include osmotic pressure, do not depend on the nature of the nonvolatile solute in the solution but rather on the concentration of solute present. Because the properties are independent of the nature of the solute, they may be used to determine other properties of the solute such as molecular weight. The effect of a nonvolatile, nonelectrolyte solute on a pure solvent will be examined by finding the freezing point depression of a cyclohexane unknown so solution. The freezing point of a pure sample of cyclohexane will be determined. Introducing a nonelectrolyte solute should in solution formed to freeze at a lower temperature. This temperature will then be experimentally determined As stated the The molality of the solution dependent on the quantity of solute particles present in solution. (moles solute particles per kilogram of solvent) is directly proportional to the change in temperature between the freezing point of the pure solvent and that of the ATfss Kim The molal freezing point depression constant, K for cyclohexane is equal to 20.00 cfmolal By experimentally determining the freezing point depression of a ohexanel unknown solution, the molal concentration is easily calculated. In turn the molar mass of the unknown olute can be calculated as well Chemistry 132 EXPERIMENT23