BIOLOGY 2B03 Lecture Notes - Lecture 13: Pilin, Cell Adhesion Molecule, Mannose
10 Apr 2018
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Lomoar cpsd| 2151316: type 1 pillus o lections on the e. coli surface recognize oligosaccharide units on the surfaces of target cells lections are located on slender hairlike appendages called fimbriae (pilli) Calnexin (protein folding chaperone) selectins (mediate cell-cell interactions) influenza haemagglutinin (viral infections) plant lectins (usually toxic: adhesin, fimh and others at the end of the pilli o fimh adhesion consists of 2 domains: Cooh-terminal pilin- involved in incorporation of. Attached fimh to latex beads and when exposed them to epithelial cells we see that these latex beads adhere to cells and are internalized: fimh is sufficient. Shown is the adhesion between a piliated bacteria and host cell. Basically measure force it takes to pull bacteria off surface. You can add inhibitors as see if force is interrupted o other assays. A= bacteria with fimh mutant= low affinity for mannose binding, doesn"t form rosettes but a pellet of rbc in bottom of the well.
