BIOLOGY 2B03 Lecture Notes - Lecture 3: Green Fluorescent Protein, Fluorescent Lamp, Electron Microscope
18 Dec 2013
School
Department
Course
Professor
Document Summary
Chapter 3: 3. 6- purifying, detecting and characterizing proteins. Molecules can be separated based on their physical or chemical characteristics. Characteristics used for separation include size, defined as either length or mass, and binding affinity for several ligands. Can separate particles and molecules that differ in mass or density. Particles in suspension with different masses or densities will settle to the bottom of the tubes at different rates; heavier particles will settle first. Resolves proteins by mass, charge or binding affinity. Based on the principle that molecules dissolved in a solution can differentially interact( bind and dissociate) with a particular solid surface. Frequent interactions will result in molecules that will spend more time bound to the surface and thus flow past the surface more slowly and vice versa. Nature of the beads in the column determines whether the separation of proteins depends on the differences in mass, charge, or binding affinity.
