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BIOLOGY 2B03 (285)
Kim Dej (39)
Lecture

Bio 2B03 guest lecture.docx

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Department
Biology
Course Code
BIOLOGY 2B03
Professor
Kim Dej

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Bio 2B03 guest lecture  Why leave the cell? • Cell signaling • Neurotransmitters (synapses, cell communication between neurons) • Cytokines, antibodies  • Insulin (made in pancreas and go to other cells to perform a function) • Hormones  Randy Schekman • work on budding yeast, creating individual mutants that cannot secret anything  (SEC mutant) Complementation test • just one gene or multiple genes that cause a certain phenotype • Pic 1: Mutant in A , not B , still have a viable cell • Pic 2: Mutant in B, not  A, still have a viable cell • Pic 3: Both mutant A and B, the cell is not viable • That suggests that both (?) or either gene A and B are required for the viability of  the cell. • Haploid yeast : sec 7 or sec 18 • Diploid yeast : sec7 + sec 18 • Haploid mate with single mutant phenotype  • sec1 (single mutant) + sec 7 (double mutant ?) >> sec 7 is upstream of sec 1.  Phenotype is of priority of sec 7 than sec 1.  BB  ▯sec 7  ▯sec 1 Where are sec genes involved?  • collagen is important in maintaining cell .. • Patients with CLSD (mutation in sec23a) have large ER but cannot perform  secretion.  James E. Rothman • SNARE proteins involved in tearing of vesicles to the membrane • important for vesicle docking • They took cells and extract the membrane fraction. They lyse open the cell and  separate proteins from membrane bound compare to cytosol and nucleus.  • Anti­Myc (antibody) epitope IgG linked to beads. They took alpha­SNAP and  gamma­SNAP. • Add NSF to bind to antibody? • They were able to find a specific receptor, discovered what 
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