BIOLOGY 2C03 Lecture Notes - Lecture 11: Tandem Repeat, Sickle-Cell Disease, Isotopic Labeling
4 May 2017
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How polymorphisms are identified in individuals using blood samples. Polymorphism must lie very close to the gene, and a particular varient near the disease causing allele (mutation in that gene). If you can find a polymorphism tightly linked to the gene, the polymorphism location will allow you to find the gene. If close, they are strongly linked and crossing over will not occur. If far away, not useful due to recombination polymorphism will dissociate from the allele. Purify the dna - treat it with enzymes, spin it down to purify. Add restriction enzyme hpa1 - involved in a polymorpsihm change - site can be there or absent in certain people. Dna was cut into millions of small pieces. Purification - run on a gel - use a probe complimentary to the sequene. Probe is radiolabelled and for a specific piece of the dna where the.
