Biology 1a03 theme 2 module 1 and 2. The central dogma: process of dna transcription mrna translation (nucleotide amino acids proteins) Transcription in prokaryotes: dna has a template strand and a non-template strand, sequence of the mrna is the same as that of the non-template strand except for the uracil replaces thymine. Initiation: rna polymerase attaches to specific promoter regions of dna, promoter regions (start of the specific gene) indicate the transcriptional starting point where. 10 region consensus sequence tataat is positioned -10 nucleotides upstream. Dna helix to allow transcription to occur: different sigma factors are able to identify specific promoters to the rna polymerase, and therefore assist with turning genes on and off when needed. Elongation: rna polymerase separate the two strands of the dna double helix, forming the transcription bubble, ribonucleotides are then able to enter the rna polymerase and assemble in a complementary fashion to the dna template strand.