BIOLOGY 1A03 Lecture Notes - Lecture 3: Reading Frame, Methionine, Smn1

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BIOLOGY 1A03 Full Course Notes
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BIOLOGY 1A03 Full Course Notes
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Open reading frame is the part of a reading frame that has the potential to code for a protein/peptide. Start at aug and stops at stop codon, with a continuous stretch of codons. In any sequence, we have 2 putative (coding) strands. Methionine (aug) sets start for reading frame and stop codon stops it. Insertion of nucleotide early is bad better after in case you reach a stop codon early, making the protein non-functional; little effect in end. Insertion/deletion in an intron has no effect bc its going to be spliced so it wont disrupt the reading frame. Insertion/deletion of 3 nucleotides (full codon) usually has no effect depending if the function of an amino acid is important and it gets removed or shifted, creating a diff amino acid (e. g. recognition) Rna could be jumping to different part of dna or rna could be being spliced! Splicing occurs at 5" end of intron first.

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