BIOLOGY 2B03 Lecture Notes - Lecture 5: Karyopherin, Guanine, Antigen
5 Dec 2017
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Nucleocytoplasmic border: nucleus anatomy, nucleolus dense spherical structure, center of nucleus, chromatin, nuclear envelope 2 lipid bilayers (inner + outer outer emerges to become er, molecules enter and exit the nucleus. John gurdon injection of histone proteins into cytoplasm: william bonner labeled nuclear proteins injected into cytoplasm. Inhibit protein synthesis and co-culture with murine cells. Induce cytoplasm fusion: a shuttling protein will relocate the murine nucleus. What kinds of proteins are transferred: nuclear localization signal (nls) allows proteins into nucleus (e. g Transcription proteins, chromosome replication proteins, ribosomal proteins: nuclear localization signal (nls) allows proteins to exit nucleus after their transcription (e. g trna, mrna, ribosomal subunits, etc. ) Importin released, ran-gdp recycled back to nucleus where guanine exchange factor (gef) exchanges the gdp with gtp. Influenza a virus capsid and genome: genome. 8 single-stranded, negative sense rna molecules (complementary to mrna: contained with viral capsid.
