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Lecture 4

BIOLOGY 2B03 Lecture Notes - Lecture 4: Green Fluorescent Protein, Pex5, Mitochondrial Matrix


Department
Biology
Course Code
BIOLOGY 2B03
Professor
Rosa Da Silva
Lecture
4

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 

TOPIC 1 – POST TRANSLATIONAL TARGETING
 !"#$
% #$  ## !"#$#&
' ()#$$(
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' + *$
' ,!()-##
.$!"#$!"#$#/#) #0!#$
12)$(#$#)#
32)#$$14
5()!#2)$(#$"
6###)!"#$#7(8
(9
,:()#
. #!"#$-; #'
"#$(##<#!#) ##
1$;!"#$#()*#!;!#'
"#$
3##!!"#$
.0)$!#($#-=#!"#$#($
6("!##$$$# #*(;-=#$$$#
>#!#!
' #<#!!
' ,!###<#
' # #$*!
' 6!4 ))##7)<$9
>' 4;)!# ?#; 
,:# !!##<&
6/3)#3@3"$6$#
o4!!
"#$!#)#
3)#3A$#-#6#
###)#! !"#$&
Hypothesis: #<#5!7#9;
 !"#$'
 B.5 ##<#A.6.4,B#!
# #C!#!&
Hypothesis: #<#)#!5 !
;;(#! !"#$'
YES ##C#<()#!
!"#$

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 
+D1,:
+D1,!#6$#:
#0)#E1 !"#$
,:# #!&
Pex50##<6$#!
( ! !"#$$$(5">$###;8#$$("
!">";F !#!"#$
o(!F$
">###;8!#$$$(!5"
,:# # &
## !"#$"$!">
o435$## #!#">G!
!"#$!"#$$"
; ">##! $"'
">###$!
Protein is now within peroxisome
">))#
,:# )<$&
""(<##$$!";8"$ #"!">
!"#$
">#(<) (<))'
H(<)<#4 ))##

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 
)$#!"#$#!
#)#!!"#$!#!;!*'
'E10!"#$#
(55'Pex$#E1$)#
#!####
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o% )##?&Also in cytosol rather than in
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$!!"#$#$!$5!#5(
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' # #
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TOPIC 2 – POST-TRANSLATIONAL TARGETING TO THE MITOCHONDRIA
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#4##) #0()!#$( $$(
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