BIOLOGY 2D03 Lecture Notes - Dna Replication Factor Cdt1, Telomerase Reverse Transcriptase, Aminoglycoside

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28 Jan 2013
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Prokaryotes
DNA Replication
Initiation: protein assembly at origin: oriC, A-T rich
Helicase – separate ds
Topoisomerase
Primase – 10bp RNA primer
Elongation: 5’-3’
Okazaki 5’-3’exonuclease: RNaseH
Termination: ter region termination region pausing at fork
Topoisomerase deconcatanate
Transcription
-operon
-promoter: Pribnow box -10, -35 sigma factor recognize specific promoter RNA Pol I
Initiation:
Promoter binding--> open complex formation -10 to +2 ternary complex: DNA, Pol,
RNA
Abortive initiation
Make 10bp sigma fall off foot print shrink 30bp
Elongation
Pausing
Driven by hydrolysis of PPi
More error prone than replication
Bridge helix
Termination
Regulated by attenuation not make unused stuff
Rho independent: GC hairping puase, Utail (AT = less stable interaction)
rho dependent: bind rut (rho utilization) hydrolysis of ATP to pull of
Translation
- 70s: 50S + 30S
- mRNA polycistronic
shine delgarno ribosome binding seq (multiple sites)
have 5’and 3’ UTR
Initiation:
Shine Delgarno 6-8 poly purine
IF1 + IF3 block other site of small subunit Psite open
mRNA bind Shine Delgarno,
fMet -tRNa bind P site at AUG
IF2 (GTPase) hydrolyze & bring in large subunit subunit joining take off initiation
factors
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