HTHSCI 4II3 Lecture Notes - Lecture 8: Cd19, Natural Killer Cell, Cd16
20 Mar 2018
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Section #1 main concepts: 3 systems of flow cytometer, fluidics: cells are required to be suspended within fluid in order to move through the cytometer, ensuring that each cell moves through one by one. By analyzing each cell individually, we can determine the cell type composition of the fluid: optics: conjugated fluorophores and antibodies are excited and emit a specific and individualized emission wavelength. It keeps lymphocytes unharmed: statement a corresponds to the definitions of fsc and ssc respectively, fsc (forward scatter) provides information on cell size (diameter and width), while ssc (side scatter) provides information on the granularity/density of the cells. Therefore, the answer is a: statement d, apc and apc efluro780 would spill significantly if you did not compensate samples, because of the strong overlap with the af700 emission spectra. Question 5: plot for the mouse histograms including nkg2d- and nkg2d+ t cell populations. s l l e.
