ISCI 1A24 Lecture Notes - Lecture 2: Nuclear Pore, Progeria, Nuclear Membrane
17 Oct 2016
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Document Summary
Nature of image depends upon type of microscope and sample prep. Resolution is the ability to distinguish between two objects in close proximity. Most important property of a microscope is the resolution not the magnification. Use uv light to illuminate sample, coupled with fluor-tagged antibodies or. Use laser beam to image a single plane within the sample. Images are taken from different focal planes and reconstructed using mathematical algorithms, generates more 3-d image. Follows electrons passing through specimen: scanning. Has higher d, follows 20 electrons emitted from surface, gives 3d sense. Nucleus: largest organelle, enclosed by double membrane, chromosomes attached to nucleus floating in space, ne studded with holes called nuclear pores, nucleolus - subnuclear structure, contains chromosome regions encoding ribosomes. *mutations in lamins cause severe laminopathies e. g hutchinson-gilford progeria. Ribosomes: millions present in cell cytosol, protein synthesis machinery, large and small subunit composed of rna & protein.
