LIFESCI 2A03 Lecture Notes - Lecture 5: Dapi, Gene Expression, Green Fluorescent Protein
23 Feb 2017
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Document Summary
Stem cell niche: a microenvironment, within the specific anatomic location where stem cells are found, which interacts with stem cells to regulate cell fate. Regulates how stem cells participate in tissue generation, maintenance and repair. Saves cells from depletion, protects the host from exuberant stem-cell proliferation. Controls signals that dictate amount of stem cells needed for organism. Each different type of stem cell has its own niche. Elements that regulate stem cell characteristics in niche. Growth factors, cytokines (molecules that help cell to cell communication) If a stem cell becomes differentiated cell, what determines its new differentiated identity. To grow stem cells in vitro we must: Culture medium bathe stem cells in liquid containing growth factors and cytokines. Feeder layer grown stem cells with other cells that supply growth factor. Make laboratory conditions similar to niche (ph, temp, etc) Somatic stem cells produce body cells for organogenesis and tissue maintenance. Germline stem cells only produce gamete for reproduction.
