LIFESCI 2A03 Lecture 5: module 2 lecture 3
1 Oct 2018
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Document Summary
Evidence from previous experiments: transfer of nuclear contents of differentiated cells to unfertilized egg (scnt) egg developed into normal organism, fusion of differentiated cells with esc (cid:1684)reversion(cid:1685) of differentiated cell to embryonic like state. Differentiated cells do have the ability to be pluripotent (even when they do not act like one) Egg/esc must contain some factors that induce pluripotency. Identifying potential reprogrammed esc-like cells: via fbx15 induced-resistance to g418 (antibiotic) Identifying the yamanaka factors: found 4 critical factors = yamanaka factors = oct . sox2, klf4, and c-myc. Assays to test pluripotency of ipscs: teratoma derived from ipscs, differentiation into multiple cell types in culture, formed normal chimeric mice after introduction into blastocyst. In vitro embryoid body formation with mef ipscs: yamanaka factors restore the ability of differentiated cells to act as pluripotent stem cells. These cells are called induced pluripotent stem cells (ipscs)
