MOLBIOL 3B03 Lecture 1: Methods
11 Sep 2016
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Dna and all genetic material is in the nucleus - have something that transfers it into the cell to change into protein. Lipid bilayer - mostly cholesterol (not really found in prokaryotic) Yeast cells are eukaryotic - more related and similar to eukaryotic systems like humans. Microscopy techniques used to study subcelllular structures and their dynamics. Light microscopy - bright field, dark field, phase contrast, dic. Fluorescence microscopy - immunocytochemistry, fluorescent probe such as gfp (live imaging), confocal imaging. Have a sample with two substances and if you have a good absorption = good contrast -> bright field. If you do(cid:374)"t ha(cid:448)e a(cid:374)y a(cid:271)sorptio(cid:374), the(cid:374) it (cid:449)ill (cid:271)e si(cid:373)ilar to the surrou(cid:374)di(cid:374)g (cid:272)ell. Phase contrast if the sample does not have a lot of absorption= phase transfer when no. Absorption - combination of dark and bright field. Incident light is in the form of a ring (east to see unstained specimen details in shades of grey)
