BCHM 316 Lecture Notes - Lecture 17: Phosphopantetheine, Transferase, Acetyl Group
1 Jun 2018
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Before the condensation reactions that build up the fatty acid
chains can begin:
TWO thiol groups that are on the enzyme complex – must be
charged with the correct acyl groups!
So like sH on Ks and sH on ACP
PRIMING STEPS
1. The acetyl group of acetyl-CoA is transferred to the ACP in
a reaction catalyzed by the malonyl/ acetyl-CoA-ACP
transferase (MAT). Afterwards, the acetyl group is then
transferred to the S-H group of β-ketoacyl-ACP synthase
(KS)
2. Malonyl group transferred from malonyl-coA to the –SH
group of ACP; this is catalyzed by malonyl/ acetyl-CoA-
ACP transferase (MAT)
FA SYNTHESIS STEPS
1. CONDENSATION
2. REDUCTION
3. DEHYDRATION
4. REDUCTION
Condensation: condensation of activated acetyl and malonyl
groups to form acetoacetyl-ACP; co2 produced; catalyzed by β-
ketoacyl-ACP synthase
Acetoacetyl-ACP : an acetoacetyl group bound to ACP through the phosphopantetheine –SH group
The acetyl group is transferred from the Cys-SH group of the
enzyme to the malonyl group on the –SH of ACP
find more resources at oneclass.com
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Document Summary
Before the condensation reactions that build up the fatty acid chains can begin: Two thiol groups that are on the enzyme complex must be charged with the correct acyl groups! So like sh on ks and sh on acp. Priming steps: the acetyl group of acetyl-coa is transferred to the acp in a reaction catalyzed by the malonyl/ acetyl-coa-acp transferase (mat). Afterwards, the acetyl group is then transferred to the s-h group of -ketoacyl-acp synthase (ks: malonyl group transferred from malonyl-coa to the sh group of acp; this is catalyzed by malonyl/ acetyl-coa- Fa synthesis steps: condensation, reduction, dehydration, reduction. Condensation: condensation of activated acetyl and malonyl groups to form acetoacetyl-acp; co2 produced; catalyzed by - ketoacyl-acp synthase. Acetoacetyl-acp : an acetoacetyl group bound to acp through the phosphopantetheine sh group. The acetyl group is transferred from the cys-sh group of the enzyme to the malonyl group on the sh of acp.
