BIOL 102 Lecture Notes - Chromatin, Microrna, Alternative Splicing

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29 Jan 2014

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Gene regulation occurs, so that energy and resources can be conserved, by only expressing certain genes during a specific situation or condition. Constitutive genes / housekeeping genes expressed continuously; needed for survival of organism. Small effector molecules binds to a regulatory transcription factor (repressor or activator) and causes a conformational change in the protein; in many cases, its effect determines whether or not the protein can bind to dna. Repressors and activators have two domains: one site at which the protein binds to the dna, binding site for the small effector molecule. Polycistronic mrna an mrna that encodes more than one protein. Lac operon contains the genes for the enzymes needed to allow it to metabolize lactose. Contains promoter, lacp, that is used to transcribe the structural genes: lacz, lacy and laca. Regulated by repressor protein (negative control) and the catabolite activator protein (cap) (positive control) cap is controlled by a small effector molecule: cyclic amp, or camp.

Related Questions

please answer all the questions answering only one question is not helpful!
Question 1

0 / 1 pts

Which of the following E. coli strains with the lac genotypes will synthesize beta-galactosidase in the presence of lactose?

Note: Superscript wouldn't work in the answers, so for example, lacIs means lacI^s(superrepressor of lacI)

lacIâ lacPâ lacOâ lacZâ lacYâ

lacIs lacP+ lacO+ lacZ+ lacYâ / lacI+ lacP+ lacO+ lacZâ lacY+

lacIs lacPâ lacO+ lacZâ lacY+ / lacI+ lacP+ lacO+ lacZ+ lacY+

lacIâ lacPâ lacO+ lacZ+ lacY+

lacI+ lacP+ lacO+ lacZ+ lacY+

Question 2

0 / 1 pts

Some mutations in the trp 5' UTR region increase termination by the attenuator. An example of a mutation that may have this effect is a

Choose the best answer!

mutation that changes the bases in region 3 of the 5' UTR, such that they no longer pair with region 2 or 4.

deletion of the two tryptophan codons in the 5' UTR leader peptide.

deletion in region 2 in the 5' UTR that prevents it from pairing with region 3.

The second and third answers are correct.

The first, second, and third answers are correct.

IncorrectQuestion 3

0 / 1 pts

Several examples of antisense RNA regulating translation in bacterial cells have been discovered. If you wanted to design a construct to artificially control translation of a bacterial gene with antisense RNA, what type of sequences should the antisense RNA contain?

A sequence complementary to the operator of an operon.

A sequence complementary to a 5' UTR.

A sequence complementary to the Shine-Dalgarno site of the mRNA.

The first and second choices are correct.

The second and third choices are correct.

Question 4

0 / 1 pts

Which of the following correctly describes the function of microRNAs?

Choose the best answer.

MicroRNAs are encoded by genes in the genome of an organism.

MicroRNAs form mRNA which encodes a protein repressor for another gene.

MicroRNAs encode a mRNA that base pairs with itself, forming double stranded RNA.

MicroRNAs are cut into small pieces, then bound by the complex of proteins called RISC to repress the translation of other mRNAs in the cell.

The first and second answers are correct.

The first, third, and fourth answers are correct.

The third and fourth answers are correct.

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BIOL 102 Lecture Notes - Chromatin, Microrna, Alternative Splicing

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