Class Notes (836,580)
Canada (509,856)
Biology (1,303)
BIOL 330 (3)
Lecture

330- Friday nov 9th, 2012- guest speaker.docx

3 Pages
98 Views
Unlock Document

Department
Biology
Course
BIOL 330
Professor
Sharon Regan
Semester
Fall

Description
330- Friday nov 9 , 2012- guest speaker - How are biopharmaceutical recombinant proteins made? o E.coli- insulin- no translational modification o Yeast- (euk) GM-CSF for bone marrow transplantation, Hirudin for anticoagulation)- hyperglycosylation o Mammalian cells- CHO cells- EPO, interferons, antibodies, etc... – expensive to produce o Transgenic plants- - Recombinant proteins in plants o Plants can process a wide variety of proteins o Free from human pathogens o Renewable source of proteins, easy to scale-up o Low cost and require limited facilities - FDA approval of first plant-made pharmaceutical- May 2 - 12:00 - Inflammatory bowel disease o Not known cause o Lesions can start from mouth to all the way down digestive tract o IL-10 protein will cause reversal of inflammation - Plant-made IL-10 is biologically active in a murine cell assay o Cells stimulated with IL-10. o By increasing IL-10 (x-axis), you get decrease in IL-6 (y axis) - Plants expressing IL10 can alleviate symptoms in a mouse model of IBD o IL10 -/- mice were fed 20% or 30% tobacco expressing IL-10 for 30 days, with ranitidine in drinking water o Global scores for ulcers, loss of crypts, infiltration and lymphoid follicles o With the C47 Black 6 mouse... we were able to alleviate the symptoms with trament of IL-10 o Not to eliminate with the C3H with IL10 in these mouses, so not as effective. It would have had been better if able to feed them more but they couldn’t - Limitations of protein production in stable transfenic plants o Speed of production  Transformationa nd regeneration of a pop (40-100)  Screening and selection of high expression  Process takes 6 months to 1 year o Accumulation levels  Low levels most of the time o 18:00 Purification from plant extracts  Colum chromatography is the most expensive step in plant-made protein production - Transient expression by Agro-infiltration o
More Less

Related notes for BIOL 330

Log In


OR

Join OneClass

Access over 10 million pages of study
documents for 1.3 million courses.

Sign up

Join to view


OR

By registering, I agree to the Terms and Privacy Policies
Already have an account?
Just a few more details

So we can recommend you notes for your school.

Reset Password

Please enter below the email address you registered with and we will send you a link to reset your password.

Add your courses

Get notes from the top students in your class.


Submit