BIOL 330 Lecture Notes - Douglas C-47 Skytrain, Vacuole, Biopharmaceutical
26 Nov 2012
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How are biopharmaceutical recombinant proteins made: e. coli- insulin- no translational modification, yeast- (euk) gm-csf for bone marrow transplantation, hirudin for anticoagulation)- hyperglycosylation, mammalian cells- cho cells- epo, interferons, antibodies, etc expensive to produce, transgenic plants- Recombinant proteins in plants: plants can process a wide variety of proteins, free from human pathogens, renewable source of proteins, easy to scale-up, low cost and require limited facilities. Fda approval of first plant-made pharmaceutical- may 2. Inflammatory bowel disease: not known cause, lesions can start from mouth to all the way down digestive tract. Plant-made il-10 is biologically active in a murine cell assay: cells stimulated with il-10, by increasing il-10 (x-axis), you get decrease in il-6 (y axis) Plants expressing il10 can alleviate symptoms in a mouse model of ibd. Il-10: not to eliminate with the c3h with il10 in these mouses, so not as effective. It would have had been better if able to feed them more but they couldn"t.
