BIOL 330 Lecture Notes - Lecture 6: Titration
26 Nov 2012
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Biol 330- lecture 5 (recording)- friday september 21st, 2012. We"re looking at transcriptional regulators, we"re looking at proteins that bind to the promoter region of dna and turn on its expression. You have yfg, want to identify what upstream region is the promoter, and you want to start to identify the protein that are transcriptional regulator of yfg. Can we identify in this experiment upstream, we want to see if we can identify dna that bind to the proteins. We isolated a piece of dna, we cut it up with restrictive enzymes, and we run it on the gel. Run that dna on gel, it migrates based on its size. Take that nuclear extract and in there, there should be proteins that bind to specific regions on that dna and trascription factors (their job is to attract the rna polymerase) Then we can see that in the gel, we can radioactively label it.
