BIOL 334 Lecture Notes - Lecture 7: Pyruvic Acid, Triglyceride, Euryhaline
25 Apr 2018
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Can lead to isolation of important genes and created improved crops. Lots of wasted time and money: targeted and purposeful alteration of specific metabolic pathways, use o regulatory properties and manipulation of specific enz, alterations can change pathway flux, target pacemaker enz and create mutant forms. Requires strong protein/ enz biochem, metabolism/ metabolic control, and physiology background. Phb = storage product of bacterium alcaligenes eutrophus: metabolic fuel that is mobilizing to produce energy for catalysis rapidly breaks down with all the attributes of pvc, good alternative to fossil fuel-derived plastics. Page 60: amplifying a flux-controlling step by circumventing a (feedback) control mechanism, and/ or by manipulating levels of allosteric effectors. [trp] is limited by trp"s feedback (allosteric) inhibition of key pacemaker. Enz of trp biosynthetic pathway = anthranilate synthase: transgenic rice/ plants achieved a high trp by encoding feedback insensitive allosteric mutant of anthranilate synthase. Production of salt-tolerant plants by engineering a protein kinase-na+ transporter.
