ENCH 213 Lecture Notes - Quantum Yield, Collaborative Product Development, Immunoassay
5 Nov 2013
School
Department
Course
Professor
Document Summary
Analysis of a mixture without separation of its components two cases: spectra of pure components have substantial overlap, regions exist where each component contributes the most, a = xb[x] + yb[y] + . For n unknowns, need at least n wavelengths to used n equations to solve for unknowns. At 510 nm, the molar absorptivities for two complexes (a and b) are 36,400 and 5250 m-1cm-1, respectively. The absorbance in a 1. 00 mm cuvet of a solution with [a] = 1. 00 x 10-4 m and [b] = 2. 00 x 10-4 m would be: 0. 469, 4. 69, 0. 780. Choose n "s where the spectrum of individual species is different (using standard solutions) Pd complex has absorption maximum at 480 nm, with = 3. 55x103 m-1cm-1: au complex has absorption maximum at 635 nm, with = 1. 45x104 m-1cm-1. Of pd at 635 nm= 5. 64x102 m-1 cm-1. Of au at 480 nm= 2. 9x103 m-1 cm-1.
