MICR 121 Lecture Notes - Lecture 20: Blood Culture, Human Microbiota, Gram Staining
14 Apr 2018
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Document Summary
Micr120 lecture 20: understanding the microbiology lab and optimizing patient care. Stands for matrix- assisted laser desorption/ ionization - time of flight spectroscopy: operates similar to mass spectrometry. Changed the way we identify organisms in microbiology. Look at proteins within an organism and blowing up the organism up. Identifies organism based on spectrum heat: relies on organism protein signatures (macromolecules) rather than metabolic activity/potential or genetic sequences. In the last decade, leaps in the ease of use of the instrument and the databases available. Clinical databases approved by regulatory bodies (fda, health canada): matrix assisted (clear glue) and laser. The matrix immobilizes and charges the sample uniformly. Optimizing blood culture on maldi-tof: mass analyzes separates molecules depending on their size. Ionizes the sample to gas phase to then be. Fast (each run of 16 organisms takes ~ 35 mins). Companies provide a database of spectra from different organisms.
