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Lecture 5

MICR 270 Lecture 5: Immune Response / Vaccines

6 Pages

Microbiology and Immunology
Course Code
MICR 270

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Module 5 20170411 3:03:00 PM Analyzing hosts immune response techniques: Enzyme linked immunosorbent (elisa) o Detects proteins, antibodies and hormones (which determine antibody concentration in diseases like HIV) o Works by producing coloured samples Inactivated virus is coated onto the wells Patient serum is introduced to the wells and if antibodies present they will bind Secondary enzymes are added to bind to the patients antibodies Substance added that binds to secondary antibodies to induce colour change Sodium dodecyl sulphate polyacrylamide gel electrophoresis (SDS Page) o Separates proteins based on weight and produce the molecular weight ladder o The smaller the peptide the further it will move Production of monoclonal antibodies o Produced by a B cell clone engineered in the lab called hybridoma which uses the cancer cells ability to replicate and the B cells antibody production to secrete large quantities of antibodies. Active Immunity Passive Immunity Natural Immunity
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