PHGY 214 Lecture Notes - Lecture 1: Axon Terminal, Chloroquine, Green Fluorescent Protein
12 May 2016
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Are there sensory neurons whose primary function is to elicit itch, but. What kind of sensory neurons have mrgpra3 mouse 1: promoter for mrgpra3 (sequence of nucleic acids) controls the expression of will be crossed (a recombination strip with cre) with mouse two: cells that contain cre. Egfp is a green fluorescent marker when looked at mouse 1 from a microscopic level, only cells that glow a fluorescent. Cq is going to be added which is a ligand for mrgpra3. All of the neurons that express mrgpra3 also express the receptor for dtr. the only place that the dtr can bind to is dtx which kills off the cell. When subjected animals to painful stimuli they appeared to be per- fectly normal. Did not matter whether the mrgpra3+ neurons were present or not behavioural consequences were 0 test by putting mice tail on hot plate and seeing how fast they re- ed spond- take it off when they perceive pain.
