BCH 261 Lecture Notes - Lecture 4: Isoelectric Point, Acid Dissociation Constant
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30 Nov 2020
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Lecture 4
● As pH drops, molecule becomes more positively charged
● As pH increases, molecule will become more negatively charged
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● Isoelectric point (pI): pH at which multiprotic acid has NO NET charge
○ pH = pKa
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○ Protein solubility is lowest when pH = pI
■ It increases when pH is higher or lower than protein’s pI
● Major Buffers