BCH 261 Lecture Notes - Lecture 4: Isoelectric Point, Acid Dissociation Constant

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30 Nov 2020
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Lecture 4
As pH drops, molecule becomes more positively charged
As pH increases, molecule will become more negatively charged
Isoelectric point (pI): pH at which multiprotic acid has NO NET charge
pH = pKa
Protein solubility is lowest when pH = pI
It increases when pH is higher or lower than protein’s pI
Major Buffers
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